目的 建立超高效液相色谱-质谱联用法测定人血浆中利奈唑胺浓度。方法 选用Waters Acquity BEH C₁₈(2.1 mm×50 mm, 1.7 μm)色谱柱,流动相为A(0.1%甲酸水溶液),B(乙腈),梯度洗脱;流速0.4 mL·min^-1;电喷雾离子源,多反应监测。利奈唑胺:⁺,m/z 338.2→296.2,卡马西平:⁺,m/z 237.1→194.2。结果 利奈唑胺在20~20 000 μg·L^-1内线性关系良好(r=0.997 9),定量下限为20 μg·L^-1。准确度与精密度结果显示方法日间、日内精密度均小于6.26%,提取回收率大于80%,稳定性较好。结论 该方法快速、灵敏、专属性强、重现性高,适用于血浆中利奈唑胺浓度测定。

OBJECTIVE To establish an UPLC-MS/MS method for the determination of linezolid in human plasma.METHODS The analytical column was packed with Acquity BEH C₁₈(2.1 mm×50 mm, 1.7 μm, Waters). The mobile phase A consisted of water(containing 0.1% formic acid), the mobile phase B consisted of acetonitrile. The analytes were eluted from the column with a linear gradient.The flow rate was 0.4 mL·min^-1. A tandem mass spectrometer equipped with electrospray ionization source was used as detector multiple reaction monitoring(MRM) using the precursor to production pairs of m/z 338.2→296.2(for linezolid)and m/z237.1→194.2(for carbamazepin) were used to quantification.RESULTS The liner range of linezolid in human plasma was 20-20 000 μg·L^-1(r=0.997 9). The limit of detection was 20 μg·L^-1. Intra-day and inter-day RSD for assaying the plasma sample of linezolid were both lower than 6.26%, absolute recovery were more than 80%.CONCLUSION The method is proved to be highly sensitive, selective, and suitable for pharmacokinetic investigations of linezolid.